Figure 5
Time response distribution of trastuzumab in relation to blood vessels and regions of hypoxia in 231-H2N xenografts. Left panels show the distribution of trastuzumab (blue) in relation to blood vessels (red) in MDA-MB-231 breast cancer xenografts transfected with ErbB2 (231-H2N) at (A) 30 min, (B) 4 h and (C) 24 h after i.v. injection of 0.3 mg trastuzumab. In right panels staining intensity due to trastuzumab is plotted against distance to the blood vessel in red and distance to region of hypoxia in green. Scale bar = 100 μm.