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Figure 1 | BMC Cancer

Figure 1

From: Prognostic relevance of ALT-associated markers in liposarcoma: a comparative analysis

Figure 1

ALT assays in liposarcomas. A) APB assay: combined PML immunofluorescence and telomere fluorescence in situ hybridization (FISH) in a frozen section of an ABP-positive liposarcoma. Indirect immunofluorescence was used for the PML protein (FITC label, green stain). Telomere FISH was done using a Cy3-conjugated telomeric peptide nucleic acid probe (red stain). Nuclei were counterstained with 4',6-diamidino-2-phenylindole (blue stain). The foci of telomeric DNA that co-localize with PML represent APB. B) TRF southern blot analysis. Telomere length distribution of a representative series of liposarcomas. The lengths of telomeres in ALT-positive cells typically range from < 3 to > 50 kb. ALT-negative cells typically have a more homogeneous distribution of telomere length and a shorter average length than ALT-positive cells.

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