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Table 2 Validation of S100A4 overexpression and copy number determination in the different cell lines.

From: Overexpression of S100A4 in human cancer cell lines resistant to methotrexate

Cell Line

Expression

  
 

Microarray

   
 

Signal Sensitive

Signal Resistant

Ratio

RT-PCR Validation

Copy- number

Protein

HT29

1876

6862

3.7

6.2 ± 0.2

1.4 ± 0.1

22.5 ± 1.0

MCF-7

49

213

4.3

6.2 ± 0.3

1.5 ± 0.1

10.8 ± 2.2

MiaPaCa-2

3304

7925

2.4

4.7 ± 0.6

1.2 ± 0.1

24.4 ± 8.1

K562

147

249

1.5

3.6 ± 0.2

1.6 ± 0.1

4.0 ± 1.0

Saos-2

137

345

2.5

2.2 ± 0.1

2.16 ± 0.07

2 ± 0.4

CaCo-2

4515

2769

0.6

N/D

N/D

N/D

MDA-MB-468

317

235

0.7

N/D

N/D

N/D

  1. The table shows the S100A4 expression levels obtained in the microarray experiments for all the cell lines studied. The level of expression is presented as the mean of the signal value in sensitive and resistant cells, as well as the ratio between them (fold change). Gene overexpression was validated in five of the seven cell lines by RT-Real-Time PCR. S100A4 gene copy number was determined by Real-Time PCR. Protein levels were determined by Western blot using a specific antibody, and quantitation was performed upon densitometry of the images. Values represent the mean (in fold change relative to each respective sensitive cells) of three experiments ± SE; N/D, non-determined.
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BMC Cancer

ISSN: 1471-2407

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