Figure 4
From: Overexpression of S100A4 in human cancer cell lines resistant to methotrexate
Effects of transfecting an expression vector encoding for β-Catenin on S100A4 mRNA levels. Transfection with β-Catenin expression vector (pcDNA3-β-Catenin) was performed in HT29 cells, both sensitive (Figure 4A) and resistant (Figure 4B) as described in Methods. S100A4 mRNA levels were determined by RT-Real-Time PCR 48 h after transfection. All results are expressed as percentages referred to untreated cells. Values are the mean of three independent experiments ± SE. * p < 0.05.