Figure 3
From: Overexpression of S100A4 in human cancer cell lines resistant to methotrexate
Effects on S100A4 expression and MTX sensitivity upon siS100A4 transfection of HT29 MTX-resistant cells. A) S100A4 mRNA levels were determined by RT-Real-time PCR as described in Methods 48 h after siS100A4 treatment. B) Cells were treated with MTX after a 48 h-pre-incubation with siS100A4, and cell viability was determined 3 days later by the MTT assay. The levels of expression and the viability results are expressed as percentages referred to the untreated cells. Values are the mean of three independent experiments ± SE. *p < 0.05, ** p < 0.01.