Role of androgen in proliferation and transcriptional activity of androgen receptor in control and Pim1-expressing LNCaP cells. (A) Effect of androgen receptor signaling on cell proliferation. Cell proliferation with or without DHT (5α-Dihydrotestosterone) treatment was analyzed in control and Pim1-expressing LNCaP cells. (B) PSA mRNA levels were measured by RT-PCR analysis after the treatment of various dose of DHT. Neo vs. Pim1 or Neo vs. K67M was compared. *P < 0.05. (C) Western blots for Pim1, androgen receptor (AR) and Actin in the indicated cell lines.