Overexpression of Pim1 in human prostate cell lines. (A) Western blots demonstrated Pim1 expression in benign human prostate cell line (RWPE1) and human prostate cancer cell lines (LNCaP and DU145). In addition, endogenous levels of c-MYC were upregulated in two cancer cell lines. Phosphorylation of p21 was increased in Pim1-expressing RWPE1 cells compared to control cells (Neo). (B) Cell sorting analyses showed that there was no difference between control (Neo) and Pim1 cells in cell cycle at the time when the cells were grafted.