Figure 4
From: Inhibition of experimental lung metastasis by systemic lentiviral delivery of kallistatin
Inhibition of angiogenesis in tumors by lentiviruses carrying kallistatin gene. Groups of mice were inoculated with LL2 cells (5 × 105) via tail vein at day 0. LV-Kallistatin or LV-GFP (108 TU), or saline were injected into mice via tail vein at day 1. (A) Lungs were excised at day 20, snap frozen and immunostaining with rabbit antibody against factor VIII-related antigen (×200). (B) Intratumoral microvessel density was determined by averaging the number of vessels in three areas of highest vessel density at × 200 magnification in each section. (mean ± SEM, n = 3 *p < 0.05, **p < 0.01, ***p < 0.001)