Figure 2

Kallistatin reduced the proliferation, migration, and invasion of LL2 cells. The CM derived from various-treated cells was diluted in serum-free medium at 1:1 and incubated with LL2 cells. (A) Cell viability was assessed by the WST-1 assay. The percentage of surviving cells (mean ± SD, n = 6) was calculated by comparing surviving cells of the CM-treated cells to saline-treated cells. Migration (B) and invasion (C) of LL2 cells (10⁴) were examined by using a 48-well Boyden chemotaxis chamber with membranes coated with gelatin (B) or collagen (C). (mean ± SD, n = 5, *p < 0.05, **p < 0.01)