Figure 1
From: Inhibition of experimental lung metastasis by systemic lentiviral delivery of kallistatin
Expression of bioactive kallistatin via lentivirus-mediated gene transfer. (A) Groups of mice were inoculated with LL2 cells (5 × 105) via tail vein at day 0. LV-Kallistatin or LV-GFP (106 TU), or saline were injected into tumor-bearing mice via tail vein at day 15. Mice were sacrificed at day 17, and tissues were harvested and measured by ELISA (mean ± SD, n = 3). The conditioned medium (CM) from LV-Kallistatin-infected TE671 cells inhibited (B) VEGF-induced and (C) bFGF-induced the proliferation of human umbilical vein endothelial cells (HUVEC). HUVEC (4 × 103/well) were treated with serum-free medium for 24 h, and then incubated with 100 μl CM in the presence of VEGF (10 ng/ml) or bFGF (15 ng/ml) for 48 h. Cell viability was measured with WST-1 assay. (mean ± SD, n = 4, * p < 0.05,**p < 0.01, ***p < 0.001)