Identification of valid reference genes for gene expression studies of human stomach cancer by reverse transcription-qPCR

Table 3 Primers for six reference genes and a target gene.

Gene	Forward primer [5'→3'] Reverse sequence [5'→3']	Anchoring Exons	Amplicon size	Spanning on genome	Amplification efficiency	Reference
ACTB	CATCGAGCACGGCATCGTCA TAGCACAGCCTGGATAGCAAC	Exon 3 Exon 4	211 bp	652 bp	1.971	[28]
GAPDH	TGCACCACCAACTGCTTA GGATGCAGGGATGATGTTC	Exon 7 Exon 8	177 bp	370 bp	1.999	[29]
HPRT1	AGACTTTGCTTTCCTTGGTCAG TCAAGGGCATATCCTACAACAA	Exon 6 Exon 8	151 bp	5120 bp	1.949	[30]
B2M	ACTGAATTCACCCCCACTGA CCTCCATGATGCTGCTTACA	Exon 2 Exon 4	114 bp	741 bp	1.924	[28]
18S rRNA	GTAACCCGTTGAACCCCATT CCATCCAATCGGTAGTAGCG	NA¹	151 bp	151 bp	2.000	[31]
RPL29	GGCGTTGTTGACCCTATTTC GTGTGTGGTGTGGTTCTTGG	Exon 1 Exon 2	120 bp	507 bp	1.937	[16]
GPNMB	TGCGTCCGTGAGAATTCA TGTGCTCCCTCATGTAAGCA	Exon 1 Exon 2	144 bp	6522 bp	1.945	In house design²

1. Not available
2. Primers were designed by Primer 3 with human mispriming library screening options.

ISSN: 1471-2407