Figure 3From: Overexpression of CDC25B, CDC25C and phospho-CDC25C (Ser216) in vulvar squamous cell carcinomas are associated with malignant features and aggressive cancer phenotypes Expression of CDC25 protein in vulvar cancer cell line. Immunohistochemical staining of CDC25A (a), CDC25B (b), CDC25C (c) and phospho-CDC25C (Ser216) (d) in CAL-39 cell line. Western blot analysis of CDC25s cellular localization in CAL-39 cells (e). Cell fractions of cytoplasm (lane 1) and nucleus (lane 2) were immunoblotted with Tubulin and Lamin B antibodies to verify the purity of subcellular fraction extraction, then with CDC25A, CDC25B, CDC25C and phospho-CDC25C (Ser216) antibodies, respectively. Cyt: Cytoplasmic fraction; Nuc: Nuclear fraction.Back to article page