Expression of CDC25 protein in vulvar cancer cell line. Immunohistochemical staining of CDC25A (a), CDC25B (b), CDC25C (c) and phospho-CDC25C (Ser216) (d) in CAL-39 cell line. Western blot analysis of CDC25s cellular localization in CAL-39 cells (e). Cell fractions of cytoplasm (lane 1) and nucleus (lane 2) were immunoblotted with Tubulin and Lamin B antibodies to verify the purity of subcellular fraction extraction, then with CDC25A, CDC25B, CDC25C and phospho-CDC25C (Ser216) antibodies, respectively. Cyt: Cytoplasmic fraction; Nuc: Nuclear fraction.