Table 2 Summary of primer sequences, used for RT-PCR, nested-PCR and MSP
Application and specificity | CpG status | Forward primer (5'-3') | Reverse primer (5'-3') | Product size (bp) | Annealing T (°C) | Ref. |
|---|---|---|---|---|---|---|
RT-PCR | ||||||
CXCL12 | - | CAACGTCAAGCATCTCAA | AGCTGCAATATCATACCGTA | 383 | 58 | This work |
GAPDH | - | CTGCACCACCAACTGCTT A | CATGACGGCAGGTCAGGTC | 296 | 63 | |
nested-PCR | ||||||
CXCL12 | - | GTAGTGAGGTTTAGTGAAG | CCATAAATACCACAATAACTTC | 479 | 51, 53, 55 | This work |
CXCL12-nested | - | AGGTTTTTGTTGGGTTGG | CAAATCCTAAATCCAACTAC | 338 | 53, 55, 57 | |
SATR-1 | - | GTTATATTATTTTTTGTTTTTTTG | ACATTTCCTTATAATATTATTCC | - | 48, 50, 52 | [21] |
SATR-1 nested | - | TATAGTGGTGGTGTATATTTG | CACCTAACCTATAATATTTCTTC | 690 | 52, 54, 56 | |
MSP | ||||||
CXCL12 - (2) | U | GAGTTTGAGAAGGTTAAAGGTTGG | CAAAAAATAAAAATACAACA | 249 | 50 | [7] |
CXCL12 - (2) | M | GTTAAAGGTCGGAGCGTATTG | ACGAAAAATAAAAATACGACGAT | 237 | 59.5 | |
CXCL12 - (4) | U | GTCGTAGCGTTGGGGTT | AACGAAACTACGCGCGACT | 200 | 57.8 | This work |
CXCL12 - (4) | M | GTTGTAGTGTTGGGGTTT | AAACAAACAAAACTACA | 189 | 64 | |
ESR1 | M | ACGAGTTTAACGTCGCGGTC | ACCCCCCAAACCGTTAAAAC | 140 | 57.6 | [20] |