Figure 1
Unsupervised hierarchical clustering of 202 tumor-specific probes (rows) in 91 CRC (columns). The 3 tumor clusters generated by this analysis were termed CIMP-High (CIMP-H), CIMP-Mid (CIMP-M) and CIMP-Low (CIMP-L). Clinicopathological and molecular features are shown above the heatmap. White rectangles are cases with missing data. Gender: female (red), male (blue); Age: ≥ 67 years (red), < 67 (blue); Tumor location: proximal (red), distal (blue); Tumor stage (ACPS): A or B (blue), C or D (red); Lymphovascular invasion (LVI): present (red), absent (blue); Extramural vascular invasion (EMVI): present (red), absent (blue); Perineural invasion (PNI): present (red), absent (blue); Tumor infiltrating lymphocytes (TILS): present (red), absent (blue); CIMPW: CIMPW-high (red), CIMPW-low (yellow), CIMPW-negative (blue); BRAF: mutant (red), wildtype (blue); KRAS: mutant (red), wildtype (blue); Microsatellite instability (MSI): positive (red), negative (blue). Five CpG clusters (A-E) were apparent from the analysis and showed differential methylation amongst the 3 CIMP subgroups.