Figure 3
From: Hypoxia increases the metastatic ability of breast cancer cells via upregulation of CXCR4
The effect of CXCR4 inhibition on cell migration (A) and invasion (B) in hypoxia-treated breast cancer cells. Human breast cancer cell line, MCF7 cells were exposed to hypoxia (2% O2 with 5% CO2) for 48 hrs, and further incubated with either a CXCR4 blocking mAb, MAB172 (mAB, 10 μg/ml), or a CXCR4 antagonist, AMD3100 (AMD, 100 nM) for 1 hr. Culture medium (CM) and control IgG2b mAb, MAB004 (IgG, 10 μg/ml) were used as the control. Cell migration and invasion were assessed as described in the Methods and expressed as (relative fluorescence units RFU). Data are presented as the mean ± SD of duplicate samples and are representative of three independent experiments. *p < 0.05 versus CM or IgG treated cells.