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Figure 1 | BMC Cancer

Figure 1

From: Griseofulvin stabilizes microtubule dynamics, activates p53 and inhibits the proliferation of MCF-7 cells synergistically with vinblastine

Figure 1

GF inhibited MCF-7 cell proliferation, arrested cell cycle progression at G2/M phase, increased the accumulation of Mad2 at the kinetochores and induced apoptosis. (A) The effect of GF on cell proliferation (closed circle) was determined by counting the cells after 48 h of incubation. The mitotic index (closed triangle) was calculated by Hoechst staining method after incubating the cells without or with GF for 24 h. Each experiment was performed four times. Data represent mean ± SD. (B) Flow cytometric analysis showing the effects of GF on the MCF-7 cell cycle (C) Effect of GF on Mad2 (green) localization. Staining was also done for a kinetochore protein Hec1 (red) (D) GF induced apoptosis in MCF-7 cells. Bars equal to 10 μm

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