Figure 4
From: Estrogen regulation of TRPM8 expression in breast cancer cells
Silencing ERα reduced TRPM8 mRNA expression. (A) Agarose gel showing ERα and TRPM8 mRNAs expression in MCF-7 cells 72 h after transfection with SiERα. (B) Histogram showing that ERα silencing reduced TRPM8 mRNA expression (**, p < 0.01, ***, p < 0.001, three independent experiments). TRPM8 and ERα mRNA levels are expressed as the ratio of TRPM8 or ERα on β-actin referent gene.