Figure 2
From: Estrogen regulation of TRPM8 expression in breast cancer cells
Icilin activated functional plasma membrane TRPM8 channel in MCF-7 breast cancer cell line. (A) Averaged I-V relationship of membrane current-densities in MCF-7 cells before and after application of Icilin (20 μM) in the bath (n = 4). Upper panel represents the patch-clamp protocol and lower panel the Icilin-activated current. Whole cell currents were elicited by a 100 ms linear ramp from -100 mV to +100 mV from a holding potential of -40 mV. (B) Average time-course of [Ca2+]c variation measured as fluorescence at 340/380 nm ratio (n = 11). (C) Average time-course of [Ca2+]c changes (n = 20) during perfusion of 20 μM Icilin and 1 μM TG in the absence of extracellular Ca2+.