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Figure 1 | BMC Cancer

Figure 1

From: Comparative proteomic analysis of plasma membrane proteins between human osteosarcoma and normal osteoblastic cell lines

Figure 1

Verification of PM through Western Blot. Fifty micrograms of homogenate, upper and down-phase proteins were separated in 11.5% SDS-PAGE and transferred to a PVDF membrane. The blots were probed with antibodies against organelle-specific proteins: anti-Na+/K+-ATPase for PM; anti-prohibitin for mitochondria. Homo, U and D mean homogenate, upper-phase and down-phase respectively. "Nor" stands for hFOB1.19 and "MG" stand for MG-63. Na+/K+-ATPase enriched upper-phase was collected as PM.

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BMC Cancer

ISSN: 1471-2407

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