Figure 4

The dynamics of tumor development in mice after ex vivo and in vivo successive treatment with siRNA and cytostatics. (A) RLS₄₀-bearing animals: wt, control mice bearing wild type tumor; luc siRNA, the mice bearing tumor cells transfected ex vivo with control luciferase siRNA; and mdr1b/1a siRNA, the mice bearing tumor cells transfected ex vivo with mdr1b/1a siRNA; (+CP) the animals treated with cyclophosphamide (100 mg/kg) on days 2 and 4 after tumor transplantation and (-CP) the animals without chemotherapeutic treatment. (B) RLS-bearing animals: wt, the control animals bearing wild type tumor; luc siRNA, the mice bearing tumor cells transfected ex vivo with control luciferase siRNA; and bcl-2 siRNA, the mice bearing tumor cells transfected ex vivo with bcl-2 siRNA; (+CP) the animals treated with cyclophosphamide (200 mg/kg) on day 2 after tumor transplantation and (-CP) the animals without chemotherapeutic treatment. (C) RLS₄₀-bearing animals: luc siRNA, the mice with tumors transfected in vivo with luciferase siRNA and mdr1b/1a siRNA, the mice with tumors transfected in vivo with mdr1b/1a siRNA; (-) the animals without chemotherapeutic treatment, (+E) the animals treated with embichin (2 mg/kg) or (+CP) cyclophosphamide (200 mg/kg) on day 2 after tumor transplantation. ** p < 0.01, compared with cytostatic treatment; ## and ###, p < 0.01 and p < 0.001, respectively, compared with wt (A) or -/luc siRNA and -/mdr1b/1a siRNA (C).