Figure 1
From: The siRNA targeted to mdr1b and mdr1a mRNAs in vivosensitizes murine lymphosarcoma to chemotherapy
Silencing of mdr1b , mdr1a , and bcl-2 genes in RLS 40 cells by siRNAs. (A) RT-PCR analysis (8% PAGE) of mdr1b, bcl-2, and mdr1a mRNA levels in the RLS40 cells transfected with control luciferase siRNA, mdr1b/1a siRNA, and bcl-2 siRNA (20-200 nM) 48 h after transfection. (B) Dose dependence of the silencing effect of gene-specific siRNAs on bcl-2, mdr1a, and mdr1b genes in RLS40 cells 48 h after transfection according to semiquantitative RT-PCR assay. The expression levels of mdr1b, mdr1a, and bcl-2 genes in the tumor cells transfected with luciferase siRNA (specific gene product/β-actin = 100%) were used as controls.