Figure 2

Gene expression of "myeloma MGF" in bone marrow subpopulations. (A) Overexpressed genes in MMC versus CD14, PMN, CD3, BMSCs and osteoclasts (p ≤ .05, fold-change ≥ 2). (B) MMC genes that were not statistically significantly overexpressed in MMC but displayed an absent call in all environment population (see additional file 2, table S-I). Histograms show the gene signal intensity on the Y axis as arbitrary units determined by the Affymetrix software. Each histogram features the same samples: 6 MB, 7 PPC, 7 BMPC, 44 MMC, 44 WBM, 5 CD3⁺/CD14⁺/PMN samples from newly diagnosed myeloma patients, 5 BMSC and 7 osteoclast samples generated in vitro. Expression data of 131 MMC samples and 75 WBM samples are presented in additional file 2. For clarity of the presentation, only 44/131 MMC and 44/75 WBM, both obtained from the same patients, are depicted here. White bars indicates that the gene displays an "absent" detection call. Abbreviations: MB, memory B cells; PPC, plasmablasts; BMPC, bone marrow plasma cells; MMC, myeloma cells, WBM, whole BM; CD3, T cells; CD14, monocytes; PMN, polymorphonuclear neutrophils; BMSC, stromal cells; OC, osteoclasts.