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Figure 6 | BMC Cancer

Figure 6

From: Interference with Activator Protein-2 transcription factors leads to induction of apoptosis and an increase in chemo- and radiation-sensitivity in breast cancer cells

Figure 6

N202.1A breast cancer cells display membrane-blebbing of upon interference with AP-2 proteins. (A) N202.1A breast cancer cells were treated with doxycycline for 96 h and Caspase 3/7 activity was quantified using an artificial Caspase 3/7 luminogenic substrate. Induced cells (+dox) expressing the dominant-negative AP-2γ mutant (Δ#7, Δ#15) display a higher Caspase 3/7 activity compared to uninduced cells (-dox) and Co#5, Co#11(-/+dox) cells. Results were derived from three independent experiments each. P-value of unpaired t-test is given (n.s. not significant). (B-G) AnnexinVC3.18-staining. N202.1A breast cancer cells are induced for 96 h using doxycycline and then stained with AnnexinV-conjugated Cy3.18 and visualized using fluorescence (B, D, E, G) or phase-contrast optics (C, D, F, G). Cells showing excessive blebbing at the membrane surface following long-term expression of the dominant-negative AP-2 mutant (B-D) are AnnexinV-positive (indicated by the arrows). Control cells treated with Staurosporine as classical inducer of apoptosis morphologically resemble the cells after interference with AP-2 function and are also AnnexinV-positive (E-G) indicating that it is an apoptosis-associated process. Scale Bar represents 10 μm.

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