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Figure 4 | BMC Cancer

Figure 4

From: Interference with Activator Protein-2 transcription factors leads to induction of apoptosis and an increase in chemo- and radiation-sensitivity in breast cancer cells

Figure 4

Interference with AP-2 results in an increase in chemo- and radiation-sensitivity in N202.1A breast cancer cells. (A) N202.1A cells were left untreated (-) or induced with doxycycline (+) for 96 h and then Adriamycin (0.3 μg/ml) was addded for 72 h (upper columns) or irradiation of 105 Gy was applied (lower columns). Visualisation under the phasecontrast microscope 72 h after treatment with Adriamycin revealed a significant increase in blebbing and dead cells in Δ#7 expressing ΔAP-2γ (+dox) compared to uninduced (-dox) and Co#11 cells (-/+dox). A decreased number of cells is also seen using Giemsa staining upon expression of ΔAP-2γ in Δ#7 (+dox) in comparison to uninduced cells and Co#5 cells. Phase contrast microscopy 72 h after irradiation revealed a higher amount of dead cells in Δ#7 compared to uninduced and Co#11 cells (-/+ dox). Lower amounts of cells are also seen using Giemsa staining upon expression of Δ#7 (+dox) in comparison to uninduced cells (-dox) and Co#11 (-/+dox). Scale Bar represents 50 μm. (B) Quantification of cell numbers shows a decrease in cell numbers of approximately 50% following interference with AP-2 proteins (Δ#7, +dox). Cell numbers are normalized to PBS treated controls. P-value of unpaired t-test is given, (n.s. not significant). (C) For determination of radiation-sensitivity the N202.1A clones are induced for 96 h or left uninduced followed by irradiation with 105 Gy. Normalisation of cell numbers to uninduced controls revealed a significant decrease in cell numbers upon expression of ΔAP-2 (Δ#7, +; Δ#15, +). P-value of unpaired t-test is given (n.s. not significant).

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