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Table 2 HPV type-specific primers and sizes of PCR-amplified fragments†

From: Detection of HPV DNA in esophageal cancer specimens from different regions and ethnic groups: a descriptive study

HPV type Primer direction†† Primer sequence Gene Size of PCR fragment (bp)
6 F 5'-CTGTTTCGAGGCGGCTATC-3' E6 323
  R 5'-TGGAGGTTGCAGGTCTAAT-3'   
16 F 5'-ATGACTTTGCTTTTCGGGATTTAT-3' E6E7 335
  R 5'-GCATGATTACAGCTGGGTTTCTC-3'   
18 F 5'-AACCGAGCACGACAGGAACG-3' E7 368
  R 5'-GGATGCACACCACGGACACA-3'   
26 F 5'-TGACCTACGCTGCTACGAACAA-3' E7 294
  R 5'-CCCGCCCCTCCTCATTT-3'   
45 F 5'-ACGACCCTACAAGCTACCAGATTT-3' E6 454
  R 5'-TTGCTATACTTGTGTTTCCCTACG-3'   
56 F 5'-TGGGGTGCTGGAGACAAACA-3' E7 271
  R 5'-CTGCACCACAAACTTACACTCACA-3'   
57 F 5'-ATACCCGAAATTGTTGACCT-3' E7 182
  R 5'-TGCTCCAGATGCCTTATGT-3'   
58 F 5'-CCAGGACGCAGAGGAGAAACC-3' E6 387
  R 5'-CGACCCGAAATATTATGAAACCTT-3'   
  1. †PCR: abbreviation for polymerase chain reaction.
  2. ††F, forward; R, reverse.
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BMC Cancer

ISSN: 1471-2407

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