Table 2 HPV type-specific primers and sizes of PCR-amplified fragmentsâ€
HPV type | Primer direction††| Primer sequence | Gene | Size of PCR fragment (bp) |
|---|---|---|---|---|
6 | F | 5'-CTGTTTCGAGGCGGCTATC-3' | E6 | 323 |
| Â | R | 5'-TGGAGGTTGCAGGTCTAAT-3' | Â | Â |
16 | F | 5'-ATGACTTTGCTTTTCGGGATTTAT-3' | E6E7 | 335 |
| Â | R | 5'-GCATGATTACAGCTGGGTTTCTC-3' | Â | Â |
18 | F | 5'-AACCGAGCACGACAGGAACG-3' | E7 | 368 |
| Â | R | 5'-GGATGCACACCACGGACACA-3' | Â | Â |
26 | F | 5'-TGACCTACGCTGCTACGAACAA-3' | E7 | 294 |
| Â | R | 5'-CCCGCCCCTCCTCATTT-3' | Â | Â |
45 | F | 5'-ACGACCCTACAAGCTACCAGATTT-3' | E6 | 454 |
| Â | R | 5'-TTGCTATACTTGTGTTTCCCTACG-3' | Â | Â |
56 | F | 5'-TGGGGTGCTGGAGACAAACA-3' | E7 | 271 |
| Â | R | 5'-CTGCACCACAAACTTACACTCACA-3' | Â | Â |
57 | F | 5'-ATACCCGAAATTGTTGACCT-3' | E7 | 182 |
| Â | R | 5'-TGCTCCAGATGCCTTATGT-3' | Â | Â |
58 | F | 5'-CCAGGACGCAGAGGAGAAACC-3' | E6 | 387 |
| Â | R | 5'-CGACCCGAAATATTATGAAACCTT-3' | Â | Â |