Figure 2

Automated Quantitative Analysis (AQUA) of Bcl-2 in lung cancer. (A-D) Tumor histospot corresponding to a NSCLC patient with Bcl-2 AQUA score of 400 A. Cytokeratin-Cy3 image used to identify tumor; the epithelial tumor "mask" is displayed in C B. Colocalization of subcellular compartments: the non nuclear (green) and nuclear (blue) compartment are defined by the cytokeratin and the DAPI signal respectively D. Bcl-2-Cy5 (red), cytokeratin (green) and DAPI (blue) colocalization; Bcl-2 shows a representative cytoplasmic pattern; AQUA score is inset into the bottom right corner.