Figure 1

Distribution and reproducibility of Bcl-2 AQUA scores in cell lines and training set. A. Plotting AQUA scores of the same histospots on serial cuts of the control array stained aside the 2 cohorts at different runs provided a standard curve in order to normalize for run to run variability (Pearson's R = o.96 between runs, p < 0.0001) B. Distribution of Ncl-2 AQUA scores in 19 cell line controls embedded in the control TMA C. Representative immunofluorescence staining in a high (MCF7) and low (SKBR3) Bcl-2 expressing breast cancer cell lines; AQUA scores are displayed in insets D. Linear regression between Bcl-2 AQUA scores of redundant tumor cores reveals significant correlation (Pearson's R = 0.85, p < 0.0001).