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Figure 2 | BMC Cancer

Figure 2

From: Elevated IGFIR expression regulating VEGF and VEGF-C predicts lymph node metastasis in human colorectal cancer

Figure 2

Induction of VEGF and VEGF-C by IGF-I in colorectal cancer cell. COLO 205 cells were incubated with various concentrations of IGF-I for 48 h at 37°C. The expression of VEGF (A) and VEGF-C (B) was analyzed by qRT-PCR in COLO 205 cells, and normalized to endogenously expressed β-actin in the same sample. VEGF (C) and VEGF-C (D) protein secretion in the cell culture supernatant was quantified using ELISA. Results are expressed as the mean ± SD of triplicate cultures and are represented of 3 independent experiments.

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BMC Cancer

ISSN: 1471-2407

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