Array-based gene expression, CGH and tissue data defines a 12q24 gain in neuroblastic tumors with prognostic implication

Table 2 Analysis of in silico gene expression data with Mann-Whitney-Wilcoxon rank sum method (MWW) revealed elevated expression of five genes at 12q24.31 amplicon.

Gene name/Genomic location	P-value for test that expression is higher in neuroblastoma than in
	healthy nervous system samples	healthy peripheral nervous system samples	all healthy tissue samples
*MYCN* /2p24.1	<1e-16	6.2e-11	<1e-16
*ALK* /2p23	<1e-16	3.6e-7	<1e-16
*MEIS1* /2p14-p13	<1e-16	9.64e-13	<1e-16
*DIABLO* /12q24.31	<1e-16	0.00302	<1e-16
*ZCCHC8* /12q24.31	<1e-16	<1e-16	1e-05
*RSRC2* /12q24.31	<1e-16	0.00031	<1e-16
*KNTC1* /12q24.31	<1e-16	<1e-16	<1e-16
*MPHOSPH9* /12q24.31	<1e-16	<1e-16	<1e-16

Gene expression levels between 22-123 neuroblastoma samples and healthy tissue samples, including 308-445 healthy nervous system (including central and peripheral nervous system samples) and 18-20 peripheral nervous system samples, were examined. In addition to the 12q24.31 amplicon genes, MYCN, ALK and MEIS1 were included in the analysis as controls. The analysis showed statistically elevated expression of DIABLO, ZCCHC8, RSRC2, KNTC1 and MPHOSPH9 in neuroblastoma samples (P < 0.01).

ISSN: 1471-2407