Figure 2

A/B: Laser scanning microscopy of adherent tumor cell (formalin fixed). After in situ perfusion with PBS with physiologic pressure through the inferior V. Cava, lungs were fixated in situ by formalin infused with physiologic pressure. After paraffin embedding, 25 μm sections were analysed by 3D laser scanning microscopy. Three dimensional reconstruction revealed tumor cells (A, yellow cell with white arrow head) adherent to the microvascular endothelium (B, white arrow heads) and leaving a perfused vessel lumen without signs of mechanical size-restricted cell arrest.