Figure 1

THL inhibits the migration and invasion of MDA-MB-231, H1299, PC-3, and CT-26 cancer cells. A) Images of wound healing assays (× 100 magnification). B) Wound healing assays indicate that THL inhibited the migration of various cancer cells dose-dependently. Values represent means ± SD, n = 3. *P < 0.05; **P < 0.001 versus untreated control. C) Boyden chamber transwell assays indicate that THL dose-dependently inhibited the migration of various cancer cells. Upper panel, images of migrated MDA-MB-231 cancer cells (× 200 magnification). Lower panel, quantitation of migrated cancer cells. The migrated cells were counted in six randomly selected microscopic fields (× 200 magnification). The relative migrated cell number was obtained by comparing the migrated cell number in the presence of THL with that in the absence of THL. Values represent means ± SD, n = 3. *P < 0.05; **P < 0.001 versus untreated control. D) THL dose-dependently inhibited the invasion of various cancer cells. The cells invaded to the lower chamber were counted in six randomly selected microscopic fields (× 200 magnification). The relative invaded cell number was obtained by comparing the invaded cell number in the presence of THL with that in the absence of THL. Value represent means ± SD, n = 2. *P < 0.05 versus untreated control.