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Table 3 Quantification cycle (Cq) values of candidate reference genes and target miRNAs (in triplicate) in colorectal tissues

From: MicroRNA expression profiling to identify and validate reference genes for relative quantification in colorectal cancer

Reference gene Cq Range Cq Min Cq Max Mean Cq ± s.d.
let-7a 8.02 24.25 32.27 28.31 ± 1.60
miR-16 4.75 21.85 26.60 23.61 ± 1.00
miR-26a 6.96 21.09 28.05 23.61 ± 1.49
miR-345 9.37 25.14 34.51 27.43 ± 1.35
miR-425 10.01 24.65 34.66 27.53 ± 1.36
miR-454 9.64 27.76 37.40 30.14 ± 1.75
RNU48 9.71 22.08 31.79 26.34 ± 2.09
Z30 12.58 26.32 38.90 31.17 ± 2.83
Target miRNA
miR-21 14.21 19.57 33.78 24.82 ± 4.07
miR-31 18.12 20.81 38.93 29.36 ± 3.75
miR-143 12.19 15.95 28.14 23.01 ± 2.84
miR-145 14.03 18.06 32.09 24.84 ± 2.79