Table 3 Quantification cycle (Cq) values of candidate reference genes and target miRNAs (in triplicate) in colorectal tissues
Reference gene | Cq Range | Cq Min | Cq Max | Mean Cq ± s.d. |
|---|---|---|---|---|
let-7a | 8.02 | 24.25 | 32.27 | 28.31 ± 1.60 |
miR-16 | 4.75 | 21.85 | 26.60 | 23.61 ± 1.00 |
miR-26a | 6.96 | 21.09 | 28.05 | 23.61 ± 1.49 |
miR-345 | 9.37 | 25.14 | 34.51 | 27.43 ± 1.35 |
miR-425 | 10.01 | 24.65 | 34.66 | 27.53 ± 1.36 |
miR-454 | 9.64 | 27.76 | 37.40 | 30.14 ± 1.75 |
RNU48 | 9.71 | 22.08 | 31.79 | 26.34 ± 2.09 |
Z30 | 12.58 | 26.32 | 38.90 | 31.17 ± 2.83 |
Target miRNA | ||||
miR-21 | 14.21 | 19.57 | 33.78 | 24.82 ± 4.07 |
miR-31 | 18.12 | 20.81 | 38.93 | 29.36 ± 3.75 |
miR-143 | 12.19 | 15.95 | 28.14 | 23.01 ± 2.84 |
miR-145 | 14.03 | 18.06 | 32.09 | 24.84 ± 2.79 |