Table 2 Details of candidate reference genes and their amplification efficiencies
Name | Length (nt) | RNA species | Accession number | Function | E (%) |
|---|---|---|---|---|---|
let-7a | 22 | miRNA | MI0000060* | Negatively regulates RAS oncogene [37] | 100.0 |
miR-16 | 22 | miRNA | MI0000070* | Negatively regulates B-cell lymphoma mRNA in chronic lymphocytic leukaemia [38] | 100.0 |
miR-26a | 22 | miRNA | MI0000083* | Involved in myogenesis and osteogenic differentiation [39, 40] | 99.8 |
miR-345 | 22 | miRNA | MI0000825* | Overexpressed in malignant mesothelioma [41] | 100.8 |
miR-425 | 22 | miRNA | MI0001448* | No functionally verified targets | 101.2 |
miR-454 | 22 | miRNA | MI0003820* | No functionally verified targets | 101.8 |
RNU48 | 63 | snoRNA | NR_002745** | Guides the 2'O-ribose methylation of 28S rRNA [42] | 100.0 |
Z30 | 97 | snoRNA | AJ007733** | Guides the methylation of the Am47 residue in U6 snoRNA [43] | 99.4 |