Figure 4From: MicroRNA expression profiling to identify and validate reference genes for relative quantification in colorectal cancer Effect of reference gene choice on relative expression of oncogenic target miRNAs in colorectal tumour (n = 35) and normal (n = 39) tissues. Boxplots depict median lines, interquartile-range boxes and outliers (*). Error bar represent range of values. Relative expression of oncogenic miRNAs: (a) miR-21 and (b) miR-31 between colorectal tumour and normal tissues normalised to different reference genes with p values indicated. The use of the two most stable reference genes: miR-16 and miR-345 detected significant dysregulation both target miRNAs between colorectal tumour and normal tissues. Dysregulation of miR-31 was observed regardless of the choice of reference indicating that it's highly differentially expressed in colorectal cancer.Back to article page