Figure 1From: MicroRNA expression profiling to identify and validate reference genes for relative quantification in colorectal cancer Relative quantity and variation associated with each candidate reference gene. (a) Quantity of candidate reference miRNAs in colorectal tumour (n = 35) and normal (n = 39) tissues as expressed as quantification cycle (Cq) values. Boxplots depict median lines, interquartile-range boxes and outliers (*). Error bars represent range of values. No significant difference (p > 0.05, t-test) was found within all reference genes between tumour and normal tissues, thus supporting further evaluation of these genes as references. (b) Variation associated with each candidate reference gene. There was a significant difference in variance (p < 0.001, Bartlett's test) associated with each reference gene indicating differing stabilities. RNU48 and Z30 showed greater variance than miR-16, miR-345 and miR-425.Back to article page