Figure 3
From: Cystatin E/M suppresses legumain activity and invasion of human melanoma
Enzyme activities of legumain (A), cathepsin B (B) and cathepsin L (C) in various cell lines. Whole cell extracts from common laboratory non-melanoma and established laboratory melanoma cell lines, primary and metastatic melanoma cell lines established from patients, and skin cell controls were collected 24 h after seeding. Legumain (A), cathepsin B (B) and L (C) activities in each cell lysate were measured by cleavage of peptide substrates and fluorometry (see Materials and methods for details). Note: The Y-axis in A and B are shown as dF/μg total protein whereas y-axis in C is shown as log dF/μg total protein.