Figure 2

Legumain, cathepsin B and L expressions in various cell lines: Common laboratory non-melanoma and established laboratory melanoma cell lines (top), and primary and metastatic melanoma cell lines established from patients, as well as skin controls (bottom). Whole cell extracts were collected 24 h after seeding and equal amounts of total protein (25 μg) were used for SDS-PAGE and immunoblotting. The filters were stained with a legumain-specific (upper panels), cathepsin B- and L-specific (middle panels) or α-tubulin specific (lower panels) antibody, respectively.