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Table 1 Apoptotic and cell cycle data for human carcinoma cell lines.

From: Normal and malignant epithelial cells with stem-like properties have an extended G2 cell cycle phase that is associated with apoptotic resistance

Cell line Treatment %CD44 high % Annexin positive cells in CD44 low % Annexin positive cells in CD44 high % cells in G2 in CD44 low % cells in G2 in CD44 high
Ca1
(n = 3)
      
  Ctrl 24 h 3.0 ± 0.2 1.4 ± 0.4 0.9 ± 0.1 28.7 ± 4.2 49.1 ± 4.9##
  5mJ UV 24 h 4.0 ± 0.2 3.3 ± 0.1* 1.1 ± 0.1# 22.5 ± 0.9 56.0 ± 4.0##
  Ctrl 48 h 3.7 ± 0.6 2.3 ± 1.0 1.0 ± 0.4# 23.6 ± 3.6 30.8 ± 1.5#
  250 μM CP 48 h 3.2 ± 0.2 5.5 ± 4.8 2.2 ± 0.5* # 54.2 ± 2.2** 68.9 ± 0.4** #
  20 ng/ml NCZ 48 h 9.1 ± 0.4* 4.9 ± 0.7* 1.6 ± 0.2# 27.2 ± 7.1 49.5 ± 2.8*
CaLH3
(n = 3)
      
  Ctrl 24 h 3.1 ± 0.3 2.8 ± 1.2 1.3 ± 0.5# 36.4 ± 2.0 42.3 ± 4.1#
  5mJ UV 24 h 3.1 ± 0.3 5.4 ± 0.9* 1.1 ± 0.2# 43.1 ± 2.4* 51.7 ± 0.4* #
  Ctrl 48 h 3.3 ± 0.6 2.7 ± 1.4 1.9 ± 0.6# 24.4 ± 9.4 34.3 ± 5.1#
  250 μM CP 48 h 4.1 ± 0.7 22.2 ± 7.8* 19.0 ± 1.8** 66.6 ± 2.8** 73.0 ± 4.1** #
  20 ng/ml NCZ 48 h 6.2 ± 0.2** 8.8 ± 1.6** 2.3 ± 0.5# 18.1 ± 0.7 40.2 ± 2.4* #
5PT
(n = 3)
      
  Ctrl 24 h 3.3 ± 0.1 1.3 ± 0.2 1.7 ± 0.4 25.6 ± 0.7 60.7 ± 5.7##
  5mJ UV 24 h 4.4 ± 0.3** 5.5 ± 2.7* 2.6 ± 1.0 37.3 ± 17.0 65.9 ± 10.0#
  Ctrl 48 h 3.1 ± 1.5 1.6 ± 0.5 1.0 ± 0.4# 18.7 ± 4.2 31.1 ± 1.9##
  250 μM CP 48 h 3.6 ± 0.9 6.3 ± 0.9** 1.8 ± 0.8# 46.0 ± 12.0 66.1 ± 2.6**
  20 ng/ml NCZ 48 h 3.4 ± 1.1 3.5 ± 0.3** 2.1 ± 0.6* # 17.1 ± 1.1 27.7 ± 2.3##
H357
(n = 3)
      
  Ctrl 24 h 4.2 ± 1.1 2.5 ± 0.9 1.4 ± 0.4# 23.6 ± 5.9 23.3 ± 3.3
  5mJ UV 24 h 3.6 ± 0.5 11.1 ± 11.6 3.6 ± 2.4# 18.3 ± 4.6 33.8 ± 15.0
  Ctrl 48 h 3.3 ± 1.0 1.3 ± 0.7 1.1 ± 0.7 19.6 ± 4.5 26.9 ± 3.2#
  250 μM CP 48 h 3.9 ± 0.3 8.7 ± 2.7** 2.1 ± 0.5* # 49.7 ± 1.6** 42.4 ± 1.6**
  20 ng/ml NCZ 48 h 7.9 ± 2.9 5.1 ± 0.8** 2.2 ± 0.8# 15.0 ± 1.0 31.5 ± 2.00#
  1. Table key: Data are presented as mean ± SD of 3 independent assays. * p value < 0.05 when compared with the control group; ** p value < 0.01 when compared with the control group; # p value < 0.05 when compared with the CD44 low population from the same group; ## p value < 0.01 when compared with the CD44 low population from the same group.
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