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Table 1 Apoptotic and cell cycle data for human carcinoma cell lines.

From: Normal and malignant epithelial cells with stem-like properties have an extended G2 cell cycle phase that is associated with apoptotic resistance

Cell line

Treatment

%CD44 high

% Annexin positive cells in CD44 low

% Annexin positive cells in CD44 high

% cells in G2 in CD44 low

% cells in G2 in CD44 high

Ca1

(n = 3)

      
 

Ctrl 24 h

3.0 ± 0.2

1.4 ± 0.4

0.9 ± 0.1

28.7 ± 4.2

49.1 ± 4.9##

 

5mJ UV 24 h

4.0 ± 0.2

3.3 ± 0.1*

1.1 ± 0.1#

22.5 ± 0.9

56.0 ± 4.0##

 

Ctrl 48 h

3.7 ± 0.6

2.3 ± 1.0

1.0 ± 0.4#

23.6 ± 3.6

30.8 ± 1.5#

 

250 μM CP 48 h

3.2 ± 0.2

5.5 ± 4.8

2.2 ± 0.5* #

54.2 ± 2.2**

68.9 ± 0.4** #

 

20 ng/ml NCZ 48 h

9.1 ± 0.4*

4.9 ± 0.7*

1.6 ± 0.2#

27.2 ± 7.1

49.5 ± 2.8*

CaLH3

(n = 3)

      
 

Ctrl 24 h

3.1 ± 0.3

2.8 ± 1.2

1.3 ± 0.5#

36.4 ± 2.0

42.3 ± 4.1#

 

5mJ UV 24 h

3.1 ± 0.3

5.4 ± 0.9*

1.1 ± 0.2#

43.1 ± 2.4*

51.7 ± 0.4* #

 

Ctrl 48 h

3.3 ± 0.6

2.7 ± 1.4

1.9 ± 0.6#

24.4 ± 9.4

34.3 ± 5.1#

 

250 μM CP 48 h

4.1 ± 0.7

22.2 ± 7.8*

19.0 ± 1.8**

66.6 ± 2.8**

73.0 ± 4.1** #

 

20 ng/ml NCZ 48 h

6.2 ± 0.2**

8.8 ± 1.6**

2.3 ± 0.5#

18.1 ± 0.7

40.2 ± 2.4* #

5PT

(n = 3)

      
 

Ctrl 24 h

3.3 ± 0.1

1.3 ± 0.2

1.7 ± 0.4

25.6 ± 0.7

60.7 ± 5.7##

 

5mJ UV 24 h

4.4 ± 0.3**

5.5 ± 2.7*

2.6 ± 1.0

37.3 ± 17.0

65.9 ± 10.0#

 

Ctrl 48 h

3.1 ± 1.5

1.6 ± 0.5

1.0 ± 0.4#

18.7 ± 4.2

31.1 ± 1.9##

 

250 μM CP 48 h

3.6 ± 0.9

6.3 ± 0.9**

1.8 ± 0.8#

46.0 ± 12.0

66.1 ± 2.6**

 

20 ng/ml NCZ 48 h

3.4 ± 1.1

3.5 ± 0.3**

2.1 ± 0.6* #

17.1 ± 1.1

27.7 ± 2.3##

H357

(n = 3)

      
 

Ctrl 24 h

4.2 ± 1.1

2.5 ± 0.9

1.4 ± 0.4#

23.6 ± 5.9

23.3 ± 3.3

 

5mJ UV 24 h

3.6 ± 0.5

11.1 ± 11.6

3.6 ± 2.4#

18.3 ± 4.6

33.8 ± 15.0

 

Ctrl 48 h

3.3 ± 1.0

1.3 ± 0.7

1.1 ± 0.7

19.6 ± 4.5

26.9 ± 3.2#

 

250 μM CP 48 h

3.9 ± 0.3

8.7 ± 2.7**

2.1 ± 0.5* #

49.7 ± 1.6**

42.4 ± 1.6**

 

20 ng/ml NCZ 48 h

7.9 ± 2.9

5.1 ± 0.8**

2.2 ± 0.8#

15.0 ± 1.0

31.5 ± 2.00#

  1. Table key: Data are presented as mean ± SD of 3 independent assays. * p value < 0.05 when compared with the control group; ** p value < 0.01 when compared with the control group; # p value < 0.05 when compared with the CD44 low population from the same group; ## p value < 0.01 when compared with the CD44 low population from the same group.