Skip to main content
Figure 1 | BMC Cancer

Figure 1

From: Epidermal growth factor induces HCCR expression via PI3K/Akt/mTOR signaling in PANC-1 pancreatic cancer cells

Figure 1

Analysis of the high specificity of the polyclonal antibody against HCCR protein by Western blotting. A) Lane 1, recombinant pMBP-c-HCCR-His protein (positive control); Lane 2-7, cell lysate from hepatocellular carcinoma tissues. Lane 2-7 showed strong bands by using the polyclonal antibody against HCCR(Mr 36,000) while they showed no bands by using the monoclonal antibody against MBP and His. B) Lane 1-5, tissues of hepatocellular carcinoma. Lane 1 showed strong band corresponding to HCCR by using the polyclonal antibody against HCCR. The band was weaken gradually and then disappeared when the antiserum was preabsorbed with gradually increased concentration of recombinant pGEM-HCCR (with a GST tag) protein from Lane 2 to Lane 5.

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us