Changes in c-myc protein expression after hypoxia in response to 1 nM, 5 mM, or 25 mM glucose. A) Western blots of c-myc and β-actin (loading control) from total cell lysates. B) Histogram of c-myc expression after 4, 6, 12, 24, or 48 h of hypoxia. Bars represent mean ± SEM expressed as a ratio to the 0 h from 3 separate experiments. * denotes a significant (p < 0.05) increase in expression and # denotes a significant decrease compared to normoxia. Note attenuation of the early increase in c-myc protein in response to 5 mM or 25 mM glucose with hypoxia. Normoxia data was previously published .