Figure 5

Effect of hypoxia on MBP-1 protein expression in response to 1 nM, 5 mM, or 25 mM glucose. A) Western blots of MBP-1 and β-actin (loading control) from total cell lysates. B) Histogram of MBP-1 expression after 4, 6, 12, 24, or 48 h of hypoxia. Bars represent mean ± SEM expressed as a ratio to 0 h from 3 separate experiments. * denotes a significant (p < 0.05) increase in expression and # denotes a significant decrease compared to normoxia. Note the brief, early increase in MBP-1 expression in cells exposed to hypoxia and 5 mM or 25 mM glucose and the brief, late increase in MBP-1 expression with hypoxia and 1 nM glucose. Overall, hypoxia attenuated MBP-1 expression at all glucose concentrations compared to normoxia. Normoxia data was previously published [26].