Hypoxia-induced changes in glut-1 mRNA in response to 1 nM, 5 mM, or 25 mM glucose at 4, 6, 12, 24, or 48 h by RT-PCR. All measurements were standardized with β-actin (loading control) and expressed as a ratio to the 0 h (expression ratio). Bars represent mean ± SEM from 3 separate experiments. * denotes a significant (p < 0.05) increase in mRNA compared to normoxia. Note early stimulation of glut-1 transcripts in response to hypoxia and the robust increase in glut-1 mRNA at 48 h with 1 nM glucose. No early increase in glut-1 transcripts occurred with normoxia. Normoxia data was previously published .