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Table 2 Primers, genomic positions, size of amplified products and annealing temperatures used in the PCR for the different components of the urokinase plasminogen activating system.

From: Increased expression of urokinase plasminogen activator and its cognate receptor in human seminomas

Gene

Primers

Exon

Size (bp)

Tann.

uPA

forward 5'-GCCATCCCGGACTATACAGA-3'

reverse 5'-AGGCCATTCTCTTCCTTGGT-3'

8

10

417

60°C

uPAR

forward 5'-CTGGAGCTGGTGGAGAAAAG-3'

reverse 5'-TGTTGCAGCATTTCAGGAAG-3'

3

5

406

60°C

PAI-1

forward 5'-ATACTGAGTTCACCACGCCC-3'

reverse 5'-GTGGAGAGGCTCTTGGTCTG-3'

3-4

5-6

320

62°C

PAI-2

forward 5'-GGCCAAGGTGCTTCAGTTTA-3'

reverse 5'-GGGATTTTGCCTTTGGTTTG-3'

2-3

5-6

384

62°C

β-Actin

forward 5'-CAAGAGATGGCCACGGCTGCT-3'

reverse 5'-TCCTTCTGCATCCTGTCGGCA-3'

3

4

275

62°C