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Figure 2 | BMC Cancer

Figure 2

From: Increased expression of urokinase plasminogen activator and its cognate receptor in human seminomas

Figure 2

Western blot analysis of uPA (A) and uPAR (C) and zymographic analysis (B) of 3 normal matched seminoma cancer tissues. Fifty μg of the different tissue protein extracts were loaded in each lane and subjected to western blot as described in the Methods section, using specific monoclonal antibodies against uPA (panel A), uPAR (panel C) and β-actin as protein loading control. In panel B, is reported the zymographic analysis of the uPA activity in protein tissues extracts of 3 normal matched seminoma cancer tissues. The conditioned medium of human breast carcinoma cell line MDA-MB-231 has been used as positive control for urokinase and tissue type PA activity, as described in the Materials and Methods section. Data reported represents one out of three similar experiments.

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