A. NO production by SL7838 cell suspensions (A
, 1.5; glucose-M9 medium) incubated without shaking in the presence of nitrate. NO concentration was determined by the oxy-haemoglobin method ; B. NO (green fluorescence) production in SL7838-infected JC cells as measured by DAF-FM diacetate. The mammalian NO synthase inhibitor, L-NAME (20 μM), was added to JC cell growth medium 24 h prior to bacterial infection. SL7838-infected JC cells were supplemented with 0.2 μM NO donor (DEA NONOate) positive control (i), 150 μM NO3
- (ii), or 2 μM NO2
- (iii), or none of these compounds (iv). Uninfected JC cells receiving 150 μM NO3
- also did not generate the fluorescence, as in (iv). The experiment was run in triplicate. Error bars represent STDEV.