Figure 1

Effect of ABZ on HIF-1α expression in vitro. Albendazole inhibits in vitro HIF-1α protein expression induced by hypoxia. OVCAR-3 cells incubated with increasing concentrations of ABZ were either kept under normoxic conditions (A) or were transferred to hypoxic chambers for 4 h (B). At the end of the experiment, whole cell extracts were examined for the expression of HIF-1α protein. Similarly, cells treated with ABZ were incubated under cell culture conditions with 100 μM hypoxia-inducing agent desferrioxamine (DFO) for 4 h (C). Each experiment was repeated at least twice. Values (mean ± s.e.m) were normalized to β-actin and the control vehicle treated cells.