Figure 3

Reduced IFN-induced P-STAT1 in melanoma cells over-expressing SOCS1 and SOCS3. Phosphorylated STAT1 was measured by flow cytometry following a 15-minute stimulation of SOCS1- and SOCS3-over-expressing cell lines with (a, b) IFN-α or (c, d) IFN-γ. Both non-transduced and empty vector transduced melanoma cell lines served as negative controls in these experiments. Error bars represent standard deviation and are derived from triplicate experiments. * Denotes p < 0.05 as compared to empty vector transduced cells.