Figure 2

Quantification of RAS mRNA expression by RT-PCR in sham livers, captopril-treated and control CRC liver metastases (tumors), and captopril-treated and control tumor-bearing liver (i.e. the liver surrounding tumors). Data for tumors is only presented at days 16 and 21 after induction as the small size of tumors was prohibitive for accurate analysis at earlier stages. Sham livers were standardized to 1 and other values normalized against sham for each time point. Significance between captopril treated and control animals is shown by connecting dotted vertical lines. The significance of other comparisons is presented in Table 2. Only ACE2 was not significantly altered either in CRC metastases or in the liver by captopril treatment.