Figure 2

SCA silencing inhibits SW780-shPSCA tumor growth. A) Balb/C nude mice were injected s.c. with SW780-shPSCA cells or SW780-shCont cells. To further lower PSCA expression a group of mice was injected with SW780-shPSCA cells pretreated with dox in vitro for 1 week (green line). Groups of 6-10 mice were injected with cells and treated as follow: SW780-shPSCA bearing mice not treated with dox (red) or treated with dox (green and blue); SW780-shCont. Treated with dox (magenta) or not treated (black). B) At the end of the experiment SW780-shPSCA tumors were collected and subjected to IHC analysis for PSCA expression (left panel) or analyzed for GFP expression (right panel).