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Table 2 Relative STAMP mRNA levels in ovarian cancer tissue culture cells after siRNA treatment

From: STAMP alters the growth of transformed and ovarian cancer cells

Cells: Hey A8 A1847 PEO-1 OVCAR10 A2780 2008 IGROV1
Percent remaining: 29 ± 5 13 ± 3 33 ± 6 25 ± 5 33 ± 9 23 ± 3 38 ± 4
Absolute STAMP mRNA relative to HeyA8): 29 ± 5 16 ± 2 47 ± 12 208 ± 37 110 ± 32 30 ± 6 43 ± 14
  1. STAMP mRNA levels in the indicated cells with transfected siRNA were determined by qRT-PCR as described in Materials and Methods and then normalized to that seen in HeyA8 cells (which was arbitrarily selected as reference) after transient transfection with Lamin siRNA. The data show both the percent remaining STAMP mRNA after siRNA treatment (i.e., 100 × STAMP siRNA/Lamin siRNA) and the "absolute" levels relative to STAMP in HeyA8 cells treated with Lamin siRNA. For this normalization to STAMP in Lamin siRNA-treated HeyA8 cells, the ratio (×100) of (STAMP mRNA in each STAMP siRNA-treated cell)/(STAMP mRNA in STAMP siRNA-treated HeyA8 cells) is multiplied by the ratio of (STAMP mRNA in STAMP siRNA-treated HeyA8 cells)/(STAMP in Lamin siRNA treated HeyA8 cells) in the same experiment (values ranged from 0.22 to 0.38). Values are the averages ± S.E.M. of 3 experiments.